Anti-Brd4 antibody (ab223223)
Key features and details
- Goat polyclonal to Brd4
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
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Overview
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Product name
Anti-Brd4 antibody
See all Brd4 primary antibodies -
Description
Goat polyclonal to Brd4 -
Host species
Goat -
Specificity
ab223223 is expected to recognize both reported isoforms (NP_490597.1; NP_055114.1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Dog, Human -
Immunogen
Synthetic peptide corresponding to Human Brd4 aa 1-100 (internal sequence) (Cysteine residue). (NP_490597.1).
Database link: O60885 -
Positive control
- WB: Mouse testis lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab223223 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 152 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 152 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Plays a role in a process governing chromosomal dynamics during mitosis. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein. -
Sequence similarities
Contains 2 bromo domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 484869 Dog
- Entrez Gene: 23476 Human
- Entrez Gene: 57261 Mouse
- Entrez Gene: 362844 Rat
- Omim: 608749 Human
- SwissProt: O60885 Human
- SwissProt: Q9ESU6 Mouse
- Unigene: 187763 Human
see all -
Alternative names
- Brd4 antibody
- BRD4-NUT FUSION antibody
- BRD4-NUT fusion oncoprotein antibody
see all
Images
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Anti-Brd4 antibody (ab223223) at 1 µg/ml + Mouse testis lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 152 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (0)
ab223223 has not yet been referenced specifically in any publications.