• Product nameAnti-Brd4 antibody
    See all Brd4 primary antibodies
  • Description
    Rabbit polyclonal to Brd4
  • Tested applicationsSuitable for: IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1312 and 1362 of human (NP_490597.1).

  • Positive control
    • Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Placenta, Prostate Carcinoma Mouse controls: Colon Carcinoma CT26, Renal Cell Carcinoma, Squamous Cell Carcinoma
  • General notes

    Concentration is optimized for IHC and not determined



Our Abpromise guarantee covers the use of ab84776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 1 µg/mg of lysate.
IHC-P 1/100 - 1/500.


  • FunctionPlays a role in a process governing chromosomal dynamics during mitosis.
  • Tissue specificityUbiquitously expressed.
  • Involvement in diseaseNote=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.
  • Sequence similaritiesContains 2 bromo domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brd4 antibody
    • BRD4-NUT FUSION antibody
    • BRD4-NUT fusion oncoprotein antibody
    • BRD4_HUMAN antibody
    • Bromodomain containing 4 antibody
    • bromodomain containing protein 4 antibody
    • Bromodomain-containing protein 4 antibody
    • CAP antibody
    • chromosome associated protein antibody
    • HUNK1 antibody
    • HUNKI antibody
    • MCAP antibody
    • Mitotic chromosome-associated protein antibody
    • Protein HUNK1 antibody
    see all

Anti-Brd4 antibody images

  • ab84776 Immunoprecipitating Brd4 in human HEK293 whole cell lysate. 1000µg of cell lysate was incubated with primary antibody (1 µg/mg lysate in 50mM TRIS) and matrix (Protein G) for 16 hours at 4°C. For western blotting ab131366 anti-mouse HRP (1/10000) was used to confirm successful immunoprecipation.


    See Abreview

  • Staining of Brd4 by Immunohistochemistry on a formalin fixed, paraffin embedded section of human colon carcinoma using a 1/500 dilution of ab84776. Detection: DAB staining
  • Staining of Brd4 by Immunohistochemistry on a formalin fixed, paraffin embedded section of mouse teratoma using a 1/100 dilution of ab84776. Detection: DAB staining

References for Anti-Brd4 antibody (ab84776)

This product has been referenced in:
  • Yoshida H  et al. Brd4 bridges the transcriptional regulators, Aire and P-TEFb, to promote elongation of peripheral-tissue antigen transcripts in thymic stromal cells. Proc Natl Acad Sci U S A 112:E4448-57 (2015). WB . Read more (PubMed: 26216992) »

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Total protein in input 1000 µg

Abcam user community

Verified customer

Submitted May 07 2014

The concentration of the Brd4 antibody ab84776 is 0.1mg/ml.