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Synthetic peptide corresponding to a region between residue 1312 and 1362 of human (NP_490597.1).
Concentration is optimized for IHC and not determined
Our Abpromise guarantee covers the use of ab84776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 1 µg/mg of lysate.|
|IHC-P||1/100 - 1/500.|
ab84776 Immunoprecipitating Brd4 in human HEK293 whole cell lysate. 1000µg of cell lysate was incubated with primary antibody (1 µg/mg lysate in 50mM TRIS) and matrix (Protein G) for 16 hours at 4°C. For western blotting ab131366 anti-mouse HRP (1/10000) was used to confirm successful immunoprecipation.
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