Anti-BRD7 antibody (ab46553)

Overview

• Product name
  Anti-BRD7 antibody
  See all BRD7 primary antibodies
• Description
  Rabbit polyclonal to BRD7
• Tested applications
  Suitable for: WBmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Chicken, Cow, Dog, Zebrafish, Rhesus monkey, Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human BRD7.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab73296.)

• Positive control
  • This antibody gave a positive signal in the following Human Whole Cell Lysates: TE 671, SW480, SHSY-5Y, HeLa - Hydroxyurea Treated (48hr, 2µM) (data not shown)

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Co-factors

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Other cell division antibodies

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Bromodomain containing

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
• Sequence similarities
  Contains 1 bromo domain.
• Cellular localization
  Nucleus.

• Target information above from: UniProt accession Q9NPI1 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 415730 Chicken
  • Entrez Gene: 29117 Human
  • Entrez Gene: 100173121 Orangutan
  • Entrez Gene: 100437229 Orangutan
  • SwissProt: Q5ZKG2 Chicken
  • SwissProt: Q9NPI1 Human
  • SwissProt: Q5R8B0 Orangutan
  • Unigene: 437894 Human
• Alternative names
  • 75 kDa bromodomain protein antibody
  • BP75 antibody
  • BRD 7 antibody

  • BRD7 antibody
  • BRD7_HUMAN antibody
  • Bromodomain containing 7 antibody
  • bromodomain containing protein 7 antibody
  • Bromodomain-containing protein 7 antibody
  • CELTIX 1 antibody
  • CELTIX1 antibody
  • NAG4 antibody
  • Protein CELTIX-1 antibody

  see all

Protocols

• Western blot protocols