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Our Abpromise guarantee covers the use of ab17969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/10000 - 1/25000. Detects a band of approximately 170 kDa (predicted molecular weight: 138 kDa).|
|IP||Use at 2-8 µg/mg of lysate.|