Overview

  • Product name
  • Description
    Rabbit polyclonal to BRD9
  • Specificity
    This antibody is specific for BRD9.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    A region within synthetic peptide: KIVANEYKSV TEFKADFKLM CDNAMTYNRP DTVYYKLAKK ILHAGFKMMS, corresponding to N terminal amino acids 73-122 of Human BRD9

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 mg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

Target

Images

  • Lane 1 : MARKER
    Lane 2 : Anti-BRD9 antibody (ab49313) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

References

ab49313 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab49313.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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