BRDG 1 overexpression 293T lysate (whole cell) (ab94204)

Overview

  • Product name
    BRDG 1 overexpression 293T lysate (whole cell)
  • Description
    BRDG 1 overexpression lysate
  • General notes
    ab94204 is a 293T cell transfected lysate in which Human BRDG 1 has been transiently over-expressed using a pCMV-BRDG 1 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: In BCR signaling, appears to function as a docking protein acting downstream of TEC and participates in a positive feedback loop by increasing the activity of TEC. Similarity: Contains 1 PH domain. Contains 1 SH2 domain. PTM: Phosphorylated on tyrosine by TEC.

Applications

Our Abpromise guarantee covers the use of ab94204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94204 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-BRDG 1 antibody (ab57031) at 1/500 dilution

    Lane 1 : BRDG 1 overexpression 293T lysate (whole cell) (ab94204)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94204 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Questions for ab94204.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up