The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 10 µg/ml.
Use a concentration of 10 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.
BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Anti-BrdU antibody [2B1] images
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [2B1] (ab115874)This image is courtesy of an Abreview submitted by Lia Schindewolffs
ab115874 staining BrdU in Mouse testis tissue, prolifierating cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with bouin, permeabilized with Tween and blocked with 20% Goat serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.