Overview

  • Product nameAnti-BrdU antibody [2B1]
    See all BrdU primary antibodies
  • Description
    Mouse monoclonal [2B1] to BrdU
  • Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Immunogen

    Chemical/ Small Molecule BrdU conjugated to Ovalbumin.

  • Positive control
    • Mouse testis tissue and Raji cells treated with BrdU.

Properties

Applications

Our Abpromise guarantee covers the use of ab115874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
Flow Cyt Use a concentration of 10 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localizationNuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody [2B1] images

  • ab115874 staining BrdU in Mouse testis tissue, prolifierating cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with bouin, permeabilized with Tween and blocked with 20% Goat serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab115874 at 10µg/ml staining BrdU in Paraffin-embedded Mouse Testis tissue by Immunohistochemistry showing incorporation of BrdU.
  • ab115874 at 10µg/ml staining BrdU in 106 BrdU treated Raji cells by Flow cytometry (shaded Histogram).

References for Anti-BrdU antibody [2B1] (ab115874)

ab115874 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
Sample Mouse Cell (Testis tissue, proliferating cells)
Specification Testis tissue, proliferating cells
Permeabilization Yes - TWEEN
Fixative Bouin
Username

Frau Dr. Lia Schindewolffs

Verified customer

Submitted Aug 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"