Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml.
ELISA Use a concentration of 1 - 0.1 µg/ml.
ICC/IF Use a concentration of 1 - 0.5 µg/ml.

Target

  • Relevance
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localization
    Nuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

Images

  • ab92837, at 5.0 µg/ml, staining BrdU by Immunohistochemistry. Image shown was generated using formalin fixation.

References

This product has been referenced in:
  • Itsara LS  et al. Oxidative stress is not a major contributor to somatic mitochondrial DNA mutations. PLoS Genet 10:e1003974 (2014). WB ; Drosophila melanogaster . Read more (PubMed: 24516391) »
  • Liu F  et al. Hydrogen sulfide improves wound healing via restoration of endothelial progenitor cell functions and activation of angiopoietin-1 in type 2 diabetes. Diabetes 63:1763-78 (2014). Read more (PubMed: 24487028) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - 0.3% triton x
Specification
heart
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 16 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6.0
Permeabilization
Yes - 0.01% Triton X-100
Specification
colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 23 2015



1.) I can confirm that I only know of few disadvantages of chicken IgY and they all do not apply to IHC:

There are some disadvantages when using certain chicken derived antibodies in immunoassays. Chicken IgY does not fix mammalian ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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