Overview

  • Product nameAnti-BrdU antibody [BRD.3], prediluted
    See all BrdU primary antibodies
  • Description
    Mouse monoclonal [BRD.3] to BrdU, prediluted
  • SpecificityReacts with BrdU in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity
    Not applicable
  • Immunogen

    Chemical/ Small Molecule corresponding to BrdU conjugated to Bovine Serum Albumin (BSA).

  • Positive control
    • Cultured cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Properties

Applications

Our Abpromise guarantee covers the use of ab74443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Target

  • RelevanceThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localizationNuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody [BRD.3], prediluted images

  • Nuclear staining of crypt epithelial cells in formalin-fixed, paraffin-embedded, BrdU-labeled mouse small intestine using ab74443 with a peroxidase-conjugated secodary antibody and a DAB chromogen.

References for Anti-BrdU antibody [BRD.3], prediluted (ab74443)

This product has been referenced in:
  • Tsutsui KM  et al. Nuclear protein LEDGF/p75 recognizes supercoiled DNA by a novel DNA-binding domain. Nucleic Acids Res 39:5067-81 (2011). Read more (PubMed: 21345933) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab74443.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"