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The details for the immunogen of this antibody are not available.
Our Abpromise guarantee covers the use of ab74545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/1 - 1/20.
Use 20ul of the suggested working dilution to label 106 cells in 100ul.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21527773|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
Detection of BrdU in cells using Rat anti BrdU:FITC (ab74545) counterstained with propidium iodide.