Anti-BrdU antibody [BU1/75 (ICR1)] (FITC) (ab74545)

Overview

  • Product name
    Anti-BrdU antibody [BU1/75 (ICR1)] (FITC)
    See all BrdU primary antibodies
  • Description
    Rat monoclonal [BU1/75 (ICR1)] to BrdU (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody recognises BrdU incorporated into single stranded DNA, attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. The antibody does not cross react with thymidine but does react weakly with chlorodeoxyuridine.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Not applicable.
  • Immunogen

    The details for the immunogen of this antibody are not available.

  • General notes

    This product will be discontinued shortly. We recommend the following product as an alternative:
    Anti-BrdU antibody (Alexa Fluor® 488) [BU1/75 (ICR1)] (ab220074)

Properties

Applications

Our Abpromise guarantee covers the use of ab74545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1 - 1/20.

Use 20ul of the suggested working dilution to label 106 cells in 100ul.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 21527773
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localization
    Nuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody [BU1/75 (ICR1)] (FITC) images

  • Detection of BrdU in cells using ab74545 counterstained with propidium iodide.

References for Anti-BrdU antibody [BU1/75 (ICR1)] (FITC) (ab74545)

This product has been referenced in:
  • Jia HY  et al. Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions. Int J Mol Med 37:359-68 (2016). IHC ; Mouse . Read more (PubMed: 26707630) »
  • Alimbetov D  et al. Suppression of the senescence-associated secretory phenotype (SASP) in human fibroblasts using small molecule inhibitors of p38 MAP kinase and MK2. Biogerontology 17:305-15 (2016). Read more (PubMed: 26400758) »

See all 10 Publications for this product

Product Wall

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I have received some further information from the originator regarding the testing of ab74545 in IHC-P:

Standard IHC-P protocols arefollowedfor this clone. However, denaturation of the ...

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