Overview

  • Product nameAnti-BrdU antibody [BU6-4]
    See all BrdU primary antibodies
  • Description
    Mouse monoclonal [BU6-4] to BrdU
  • SpecificityThis antibody specifically reacts with bromodeoxyuridine.
  • Tested applicationsSuitable for: ELISA, Flow Cyt, WBmore details
  • Immunogen

    Bromodeoxyuridine-BSA conjugate.

Properties

Applications

Our Abpromise guarantee covers the use of ab12219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Use under non reducing condition.

Target

  • RelevanceThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localizationNuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

References for Anti-BrdU antibody [BU6-4] (ab12219)

This product has been referenced in:
  • Irwin RW  et al. Allopregnanolone preclinical acute pharmacokinetic and pharmacodynamic studies to predict tolerability and efficacy for Alzheimer's disease. PLoS One 10:e0128313 (2015). Read more (PubMed: 26039057) »
  • Singh C  et al. Allopregnanolone restores hippocampal-dependent learning and memory and neural progenitor survival in aging 3xTgAD and nonTg mice. Neurobiol Aging 33:1493-506 (2012). Flow Cyt . Read more (PubMed: 21803451) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"