• Product nameAnti-BrdU antibody [IIB5]
    See all BrdU primary antibodies
  • Description
    Mouse monoclonal [IIB5] to BrdU
  • SpecificityBrdU is a thymidine analogue and when offered to proliferating cells it is incroporated into reduplicating cells. The antibody is specific for DNA in which BrdU has been incorporated. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In immuncytochemistry the antibody only recognizes BrdU in denaturated (single stranded) DNA. The BrdU antibody is 100% crossreactive with Iodo-Deoxy-Uridine (IrdU). Therfore, IdU instead of BrdU can be used in studies.
  • Tested applicationsSuitable for: IHC-FoFr, ICC/IF, Flow Cyt, IHC-Fr, ICC, IHC-Pmore details
  • Immunogen

    Chemical/ Small Molecule BrdU conjugated to BSA.

  • Positive control
    • Bromodeoxyuridine labeled cells.



Our Abpromise guarantee covers the use of ab8152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/5 - 1/10.

Dilute antibody in 0.15 M phosphate buffered saline with 1% BSA and 1% Na-azide.

ICC Use at an assay dependent concentration.
IHC-P 1/5 - 1/10. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Enzymatic antigen retrieval with proteases can also be used.


  • RelevanceThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localizationNuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody [IIB5] images

  • ab8152 (1/100) staining BrdU in HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further esperimental details please see Abreview.

    See Abreview

  • Immunohistochemical analysis of rat brain tissue, staining BrdU with ab8152.

    Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/20 in diluent) for 18 hours at 25°C. A Cy3®-conjugated donkey anti-mouse polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Cells were pulse labeled with 10 mM BrdU for 30 min, rinsed twice in prewarmed PBS, and chased in prewarmed culture medium, supplemented with 5 mM deoxythymidine. Incorporated BrdU was detected after ethanol fixation of the cells, which were than rinsed once in PBS and resuspended in 2 ml of 0.4 mg/ml pepsin in 0.1 N HCl. After 30 min at room temperature cells were pelleted, resuspended in 2 N HCl, and incubated for another 30 min at 37°C. Cells were rinsed in 0.1 M borate buffer, pH 8.5, and PBS/BSA (1 mg/ml BSA in PBS). Appropriately diluted mouse anti-BrdU antibody (clone IIB5) was added to the cell pellet, resuspended in 100 micro liters PBS/BSA. After incubation for 1 h at room temperature, the cells were rinsed twice in PBS/BSA. For visualization, FITC-conjugated Fab2 fragments of rabbit anti-mouse Ig antibody were added in a 1/10 dilution. After incubation for 45 min at room temperature samples were rinsed twice in PBS/BSA and the cells were finally resuspended in 0.5 ml cold PBS supplemented with 100 microgram/ml RNAse and 20 µg/ml propidium iodide. The samples were allowed to stand for 15 min on ice in the dark before flow cytometric analysis. In the negative control the primary antibody was omitted.

References for Anti-BrdU antibody [IIB5] (ab8152)

This product has been referenced in:
  • Taylor SR  et al. Neuroblast Distribution after Cortical Impact Is Influenced by White Matter Injury in the Immature Gyrencephalic Brain. Front Neurosci 10:387 (2016). Read more (PubMed: 27601978) »
  • Yu HP  et al. TIGAR regulates DNA damage and repair through pentosephosphate pathway and Cdk5-ATM pathway. Sci Rep 5:9853 (2015). Read more (PubMed: 25928429) »

See all 6 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Sample Human Cell (OCI ly1)
Specification OCI ly1
Permeabilization Yes - 0.25% Triton X-100 in PBS
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 07 2014

Thank you for your inquiry and for rating your experience with us.

I just wanted to follow up on your query with some additional information.

For ab8039, this antibody can be used for detection of BrdU and BrU. We have not tested it...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 07 2012

Thank you for contacting us.

For floating sections, please check thefollowing protocol on IHC World website:
This protocol is for paraffin-embedded as ...

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Thank you for your email.

The slides need to be immersed, for the retrieval to be effective. Yes, the sections could come off ifthey are kept too long in the retrieval solution. Should they come off, you would need to reduce the time they ar...

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Thank you for your email.

To answer your questions:

1) If you use a fluorescent secondary antibody, then steps 15-18 can be left out. You would do step 19 however, using DAPI or Hoechst as counterstain.

2) Incubation time...

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Thank you for contacting us.

I have checked with the lab:
Antigen retrieval can be done with HIER using citrate buffer.

Please see below for the protocol:

High Temperature antigen Unmasking Technique using Sodium Cit...

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Thank you for your reply.

I am currently trying to find a BrdU antibody that we know will only recognize BrdU. I will let you know once I find a suitable antibody.

In the meantime, please let me know if there is anything else I can ...

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Thank you for contacting us with your question. We unfortunately do not have any data regarding the minimum length of ssDNA required for antibody staining on adherent cells. I am sorry that this characterization has not been performed and that we don't...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (Oligodendrocytes, astrocytes (mixed glial culture))
Specification Oligodendrocytes, astrocytes (mixed glial culture)
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton-PBS for 30 minutes followed by DNAse treatment for 90 minutes.

Abcam user community

Verified customer

Submitted Oct 28 2010