• Product nameAnti-BrdU antibody [IIB5]
    See all BrdU primary antibodies
  • Description
    Mouse monoclonal [IIB5] to BrdU
  • SpecificityThis antibody also reacts with bromodeoxyuridine when incorporated into nuclear DNA. It cross reacts with iododeoxyuridine (IdU).
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, ICC, IHC-Pmore details
  • Immunogen

    Chemical/ Small Molecule conjugated to BSA.



Our Abpromise guarantee covers the use of ab8955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/100 - 1/200. ABC method.
ICC Use at an assay dependent concentration. Using immunocytochemistry, a combination of this antibody and Mouse monoclonal to Mitotic Cells (ab8956) can distinguish and quantitate the four major fractions of the cell cycle.
IHC-P 1/100 - 1/200. ABC method.


  • RelevanceThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localizationNuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

References for Anti-BrdU antibody [IIB5] (ab8955)

This product has been referenced in:
  • Birey F & Aguirre A Age-Dependent Netrin-1 Signaling Regulates NG2+ Glial Cell Spatial Homeostasis in Normal Adult Gray Matter. J Neurosci 35:6946-51 (2015). IHC-FrFl ; Mouse . Read more (PubMed: 25926469) »
  • Tsihlis ND  et al. Nitric oxide is less effective at inhibiting neointimal hyperplasia in spontaneously hypertensive rats. Nitric Oxide 35:165-74 (2013). IHC-Fr . Read more (PubMed: 24149190) »

See all 6 Publications for this product

Product Wall

We unfortunately do not have any data confirming its cross reactivity with chlorodeoxyuridine. We however have customer feedback that say it does not bind to CldU or Idu but there is no tested data available. In case you are interested in buying and...

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Vielen Dank für Ihren wirklich sehr netten Anruf und für Ihr Interesse an unseren Produkten. Unseres Wissens nach wurde der Antikörper ab8955 bisher noch nicht in IHC-FrFl getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein s...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cultured Cells (BMSC)
Specification BMSC
Fixative Paraformaldehyde
Permeabilization Yes - Triton X100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

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Submitted Apr 22 2008