Anti-BrdU antibody [MoBu-1] (ab8039)
Key features and details
- Mouse monoclonal [MoBu-1] to BrdU
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-BrdU antibody [MoBu-1]
See all BrdU primary antibodies -
Description
Mouse monoclonal [MoBu-1] to BrdU -
Host species
Mouse -
Specificity
This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. The reaction shows a clear, nuclear confined speckled pattern. It reacts also specifically with 5-bromouridine (BrU). -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to BrdU.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
>95 % (by PAGE). -
Clonality
Monoclonal -
Clone number
MoBu-1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 2 µg/ml.
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Flow Cyt (Intra) |
Use a concentration of 1 - 2 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 2 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 1 - 2 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
Cellular localization
Nuclear -
Alternative names
- Bromodeoxyuridine antibody
- BUdr antibody
Images
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Immunohistochemical analysis of parafin-embedded bromodeoxyuridine-labelled chick embryo cells with (MoBu-1) 5-bromodeoxyuridine
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ICC/IF image of ab8039 stained HeLa cells, both BrdU treated (left image) and normal cells (right image). The cells were 100% methanol fixed (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8039, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Positive staining can be seen in the BrdU treated cells, but not in the normal cells, demonstrating specificity for BrdU.
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IHC image of ab8039 staining, both in normal and BrdU treated rat liver formalin fixed paraffin embedded tissue sections, performed on a Leica Bond
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Intracellular Flow Cytometry analysis of CEM (human acute lymphoblastic leukemia) cells labelling BrdU with ab8039 at 1µg/mL. Goat anti-mouse IgG was used as the secondary antibody.The individual cell cycle phases (S, G1, G2/M-phase) are indicated on the figure.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab8039 has been referenced in 28 publications.
- Xie Z & Bankaitis VA Phosphatidylinositol transfer protein/planar cell polarity axis regulates neocortical morphogenesis by supporting interkinetic nuclear migration. Cell Rep 39:110869 (2022). PubMed: 35649377
- Elango R et al. The structure-specific endonuclease complex SLX4-XPF regulates Tus-Ter-induced homologous recombination. Nat Struct Mol Biol 29:801-812 (2022). PubMed: 35941380
- Chiang SY et al. Usp11 controls cortical neurogenesis and neuronal migration through Sox11 stabilization. Sci Adv 7:N/A (2021). PubMed: 33579706
- Whelan DR & Rothenberg E Super-resolution mapping of cellular double-strand break resection complexes during homologous recombination. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33707212
- Wayne J et al. Targeting DNA damage response pathways to activate the STING innate immune signaling pathway in human cancer cells. FEBS J 288:4507-4540 (2021). PubMed: 33529438