• Product name
    Anti-BrdU antibody [MoBu-1]
    See all BrdU primary antibodies
  • Description
    Mouse monoclonal [MoBu-1] to BrdU
  • Specificity
    This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. The reaction shows a clear, nuclear confined speckled pattern. It reacts also specifically with 5-bromouridine (BrU).
  • Tested applications
    Suitable for: ICC, IHC-P, ICC/IF, Flow Cytmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to BrdU.



Our Abpromise guarantee covers the use of ab8039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 2 µg/ml.
Flow Cyt Use a concentration of 1 - 2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Relevance
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localization
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody


  • ICC/IF image of ab8039 stained HeLa cells, both BrdU treated (left image) and normal cells (right image). The cells were 100% methanol fixed (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were  then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8039, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Positive staining can be seen in the BrdU treated cells, but not in the normal cells, demonstrating specificity for BrdU.

  • IHC image of ab8039 staining, both in normal and BrdU treated rat liver formalin fixed paraffin embedded tissue sections, performed on a Leica Bond

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow cytometry analysis of CEM (human acute lymphoblastic leukemia) cells labelling BrdU with ab8039 at 1 µg/mL. Goat anti-mouse IgG was used as the secondary antibody. The individual cell cycle phases (S, G1, G2/M-phase) are indicated on the figure. 


This product has been referenced in:
  • Li L  et al. SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability. Nat Commun 7:12235 (2016). Flow Cyt . Read more (PubMed: 27436229) »
  • Zhang W  et al. MiRNA-128 regulates the proliferation and neurogenesis of neural precursors by targeting PCM1 in the developing cortex. Elife 5:N/A (2016). Read more (PubMed: 26883496) »

See all 9 Publications for this product

Customer reviews and Q&As

Thank you for your inquiry and for rating your experience with us.

I just wanted to follow up on your query with some additional information.

For ab8039, this antibody can be used for detection of BrdU and BrU. We have not tested it...

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Thank you for contacting us. We have not tested these antibodies for any cross-reactivity with IdU. Please let me know if you have any further questions.

Thank you for your reply.

We know of one publication at this moment in time where this antibody was in flow cytometry (also stated in the reference tab of the datasheet):

http://www.ncbi.nlm.nih.gov/pubmed/18625847?dopt=Abstract Read More

Thank you for your inquiry.

I can confirm that ab8039 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle.

It does not matter what species the DNA is from. If BrdU is incorporated into human or ovine D...

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Yes. Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail.


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