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Our Abpromise guarantee covers the use of ab1893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FrFl||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 10 µg/ml.|
|IHC-Fr||Use a concentration of 10 µg/ml.|
|IP||Use a concentration of 25 - 100 µg/ml.|
|ICC/IF||Use at an assay dependent dilution. PubMed: 21118958|
|ELISA||Use at an assay dependent dilution.|
|IHC-FoFr||Use at an assay dependent dilution. PubMed: 19332057|
ab1893 at a 1/50 dilution staining rat brain tissue sections by IHC-P. The incubation time with the primary antibody was overnight at 4°C. This image shows double-staining by polyclonal sheep BrdU (labeled with Cy3 - red) and nestin 401v (labeled with Alexa Fluor® 488 - green). The detection of proliferative (BrdU-positive) astrocytes (some of them express also nestin, which demonstrates that they are reactive and of lower differentiation compared with later stages of reactive astrocytes) within the rat striatum 6 days after striatal neurotoxic lesion (by quolinic acid).
ab1893 Immunofluorescence data
Tissue culture cells were labelled prior to transplantation and then identified in in vivo tissue using ab1893 sheep polyclonal BrdU antibody (10ug/ml incubated overnight at room temperature) with a TRITC conjugated secondary antibody.
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