Anti-BrdU antibody - Proliferation Marker (ab1893)

Overview

  • Product nameAnti-BrdU antibody - Proliferation MarkerSee all BrdU primary antibodies ...
  • Description
    Sheep polyclonal to BrdU - Proliferation Marker
  • Tested applicationsIHC-FrFl, IHC-P, IHC-Fr, IP, ICC/IF, ELISA, IHC-FoFrmore details
  • Immunogen

    Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH).

Properties

Applications

Our Abpromise guarantee covers the use of ab1893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
IP Use a concentration of 25 - 100 µg/ml.
ICC/IF Use at an assay dependent dilution. PubMed: 21118958
ELISA Use at an assay dependent dilution.
IHC-FoFr Use at an assay dependent dilution. PubMed: 19332057

Target

  • RelevanceThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localizationNuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody - Proliferation Marker images

  • ab1893 used in IP.
  • ab1893 at a 1/50 dilution staining rat brain tissue sections by IHC-P. The incubation time with the primary antibody was overnight at 4°C. This image shows double-staining by polyclonal sheep BrdU (labeled with Cy3 - red) and nestin 401v (labeled with Alexa Fluor® 488 - green). The detection of proliferative (BrdU-positive) astrocytes (some of them express also nestin, which demonstrates that they are reactive and of lower differentiation compared with later stages of reactive astrocytes) within the rat striatum 6 days after striatal neurotoxic lesion (by quolinic acid).

    See Abreview

  • ab1893 Immunofluorescence data

    Tissue culture cells were labelled prior to transplantation and then identified in in vivo tissue using ab1893 sheep polyclonal BrdU antibody (10ug/ml incubated overnight at room temperature) with a TRITC conjugated secondary antibody.

References for Anti-BrdU antibody - Proliferation Marker (ab1893)

This product has been referenced in:
  • Yang L  et al. Electroacupuncture promotes proliferation of amplifying neural progenitors and preserves quiescent neural progenitors from apoptosis to alleviate depressive-like and anxiety-like behaviours. Evid Based Complement Alternat Med 2014:872568 (2014). IHC-FrFl . Read more (PubMed: 24719647) »
  • Lee CC  et al. Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans. Cell Death Dis 5:e1158 (2014). Read more (PubMed: 24722283) »

See all 34 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample African Green Monkey Cultured Cells (COS7)
Specification COS7
Permeabilization Yes - 0.5% TritonX100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 27 2014

Thank you for contacting us.

Unfortunately we do not have any cross-reactivity data against FDU either.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your telephone call yesterday afternoon.

I have received some further information regarding the protocol for use with ab1893 BrdU antibody.

There are two protocols we are aware of that have been used with this antibo...

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Thank you for contacting us. Cultured cells should be pulsed with 10uM BrdU for 1 hour - overnight. I hope this helps, please let me know if you need any additional information or assistance.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (colon cancer)
Specification colon cancer
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 07 2012

T
Thank you for your patience in this matter.



I have been trying to determine if any of our BrdU antibodies are only specific to BrdU or not and it seems that most of them have some sort of cross reactivity with either ErdU or ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Paraformaldehyde fixed mouse brain sections)
Specification Paraformaldehyde fixed mouse brain sections
Fixative No
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 19 2012

Thank you for contacting us.

We have checked the instock vials and can confirm that the liquid is frozen at -20C. You may need to check the freezer temperature because the antibody buffer should get frozen at -20C.

There is also n...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry - Free Floating
Sample Rat Tissue sections (Brain section)
Specification Brain section
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Nov 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"