Anti-BrdU antibody - Proliferation Marker (ab1893)

Overview

  • Product name
    Anti-BrdU antibody - Proliferation Marker
    See all BrdU primary antibodies
  • Description
    Sheep polyclonal to BrdU - Proliferation Marker
  • Tested applications
    Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ICC/IF, ELISA, WB, IHC-FoFrmore details
  • Immunogen

    Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)

Properties

Applications

Our Abpromise guarantee covers the use of ab1893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21118958
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19332057

Target

  • Relevance
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localization
    Nuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

Images

  • ab1893 staining BrdU in COS7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 2% BSA) for 1 hour at 25°C. ab96945, a DyLight® 594-conjugated rabbit anti-sheep IgG (H+L) polyclonal was used as the secondary antibody (1/200). Nuclei are stained blue with DAPI.

    See Abreview

  • ab1893 staining BrdU in Ramos cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Kim SH  et al. Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity. Oncotarget 8:5026-5037 (2017). ICC/IF ; Human . Read more (PubMed: 27926513) »
  • Xie Q  et al. Folate deficiency facilitates recruitment of upstream binding factor to hot spots of DNA double-strand breaks of rRNA genes and promotes its transcription. Nucleic Acids Res 45:2472-2489 (2017). Read more (PubMed: 27924000) »

See all 76 Publications for this product

Customer reviews and Q&As

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ramos cell line xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization
No
Specification
Ramos cell line xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 25 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Aug 25 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
African Green Monkey Cultured Cells (COS7)
Specification
COS7
Permeabilization
Yes - 0.5% TritonX100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 27 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (colon cancer)
Specification
colon cancer
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Jun 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Paraformaldehyde fixed mouse brain sections)
Specification
Paraformaldehyde fixed mouse brain sections
Fixative
No
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Apr 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain section)
Specification
Brain section
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Nov 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Mammary epithelial MCF10A)
Specification
Mammary epithelial MCF10A
Fixative
3% paraformaldehyde then 100% methanol
Permeabilization
Yes - 2M HCL (30mins RT) for DNA denaturation
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Dr. Jennifer Anderson

Verified customer

Submitted Apr 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 23 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
2%Blockace/0.05% Triton X-100/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain: dentate gyrus of Hippocampus)
Specification
Brain: dentate gyrus of Hippocampus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Modified Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted May 30 2008

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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