Anti-BrdU antibody - Proliferation Marker (ab1893)

Overview

  • Product name
    Anti-BrdU antibody - Proliferation Marker
    See all BrdU primary antibodies
  • Description
    Sheep polyclonal to BrdU - Proliferation Marker
  • Tested applications
    Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ICC/IF, ELISA, WB, IHC-FoFrmore details
  • Immunogen

    Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)

Properties

Applications

Our Abpromise guarantee covers the use of ab1893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21118958
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19332057

Target

  • Relevance
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localization
    Nuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

Images

  • ab1893 staining BrdU in COS7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 2% BSA) for 1 hour at 25°C. ab96945, a DyLight® 594-conjugated rabbit anti-sheep IgG (H+L) polyclonal was used as the secondary antibody (1/200). Nuclei are stained blue with DAPI.

    See Abreview

  • ab1893 staining BrdU in Ramos cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Kim SH  et al. Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity. Oncotarget 8:5026-5037 (2017). ICC/IF ; Human . Read more (PubMed: 27926513) »
  • Xie Q  et al. Folate deficiency facilitates recruitment of upstream binding factor to hot spots of DNA double-strand breaks of rRNA genes and promotes its transcription. Nucleic Acids Res 45:2472-2489 (2017). Read more (PubMed: 27924000) »

See all 76 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Unfortunately we do not have any cross-reactivity data against FDU either.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your telephone call yesterday afternoon.

I have received some further information regarding the protocol for use with ab1893 BrdU antibody.

There are two protocols we are aware of that have been used with this antibo...

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Thank you for contacting us. Cultured cells should be pulsed with 10uM BrdU for 1 hour - overnight. I hope this helps, please let me know if you need any additional information or assistance.

T
Thank you for your patience in this matter.



I have been trying to determine if any of our BrdU antibodies are only specific to BrdU or not and it seems that most of them have some sort of cross reactivity with either ErdU or ...

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Thank you for contacting us.

We have checked the instock vials and can confirm that the liquid is frozen at -20C. You may need to check the freezer temperature because the antibody buffer should get frozen at -20C.

There is also n...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Thank you for your enquiry. Unfortunately this antibody has not been tested against IDU.

Thank you for your enquiry and your interest in our products. The Brdu antibody should recognize both incorporated and free Brdu. The free Brdu should wash away in the experiment. I hope this information will be useful.

Thank you for your email and your kind words about Abcam. Unfortunately we cannot match our competitor's price. We spend alot of time calculating the amount of antibody we can sell for a particular price based not only on the cost of bringing the ant...

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Thank you for contacting your customer and for providing further information about the staining procedure. I have read through all the messages regarding this enquiry, and as I understand this troubled customer has tried to follow the method we have...

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1-10 of 19 Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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