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ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.
The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.
Control Slides: Mouse intestinal tissue pulsed with BrdU ab129956
*Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution)) is not provided in this kit.
|4X Trypsin Enzyme Concentrate*||1 x 3ml|
|Blocking Buffer||1 x 6ml|
|Control Slides||5 slides|
|DAB Concentrate||1 x 0.3ml|
|Denaturing Solution||1 x 6ml|
|Detector Antibody (Biotinylated and pre-diluted)||1 x 6ml|
|Hematoxylin Counterstain||1 x 6ml|
|Mounting Media||1 x 6ml|
|Streptavidin-HRP Conjugate (pre-diluted)||1 x 6ml|
|Substrate Reaction Buffer||1 x 6ml|
|Trypsin Dilution Buffer*||1 x 12ml|
Our Abpromise guarantee covers the use of ab125306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|