Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR) (ab104827)

Overview

  • Product nameBreast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR)
  • Description
    Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR)
  • Product overviewab104827 is a human breast cancer biomarker panel containing 50µg of Ki67 rabbit polyclonal, 50µg of estrogen receptor alpha rabbit polyclonal, 50µg of ErbB 2 rabbit polyclonal, 50µg of p53 mouse monoclonal and 50µg of progesterone receptor mouse monoclonal antibodies. This panel contains a selection of biomarkers used to identify breast cancer cells. Much interest has been focused on cancer biomarkers, as recent studies have shown that they are useful for predicting cancer disease progression.
    The components of this product are:
    ab15580 Rabbit polyclonal to Ki67 - Proliferation Marker 50µg Concentration: Lot specific Applications: Flow Cyt: 1/100 ICC/IF: 1/100 - 1/1000 IHC-P: 1/200 IHC-Fr: 1/100-1000 IHC-FoFr: Use at an assay dependent dilution IHC-FrFl: 1/50 WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 345, 395 kDa (predicted molecular weight: 359 kDa)
    ab37438 Rabbit polyclonal to Estrogen Receptor alpha 50µg Concentration:1 mg/ml Applications: ICC/IF: Use at a concentration of 5 µg/ml IHC-P: Use at a dilution of 1/100 WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 68 kDa
    ab2428 Rabbit polyclonal to ErbB 2 250µl Concentration: 0.2 mg/ml Applications: IHC-P: Use at an assay dependent dilution IHC-Fr: Use at an assay dependent dilution IP: Use at an assay dependent dilution WB: 1/200
    ab26 Mouse monoclonal [PAb 240] to p53 50µg Concentration: Lot specific Applications: ELISA: 1/100 Flow Cyt: Use at an assay dependent dilution ICC/IF: Use at an assay dependent dilution IHC-P: 1/250 - 1/500 IHC-Fr: 1/250 - 1/500 IHC (Methanol fixed): 1/250 IP: Use at 10µg/mg of lysate WB: 1/1000 - 1/2000. Detects a band of approximately 53 kDa
    ab2764 Mouse monoclonal [PR-AT 4.14] to Progesterone Receptor - ChIP Grade 50µg Concentration:1 mg/ml Applications: ChIP: Use at an assay dependent dilution. (PubMed: 19553525) ICC/IF: 1/500. Using T47D cells. IHC-P: Use at a concentration of 5 µg/ml. IHC-Fr: Use at a concentration of 5 µg/ml. Immunohistochemical staining of PR in rat uterus with this antibody yields intense nuclear staining. IP: Use at an assay dependent dilution. WB: Use at a concentration of 5 µg/ml. By Western blot, this antibody detects a 94 kDa and a 120 kDa protein representing PRA and PRB, respectively from rat uterine homogenate. Predicted molecular weight: 99 kDa. (PubMed: 16912069)
    Storage Instructions Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Components

      Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR) images

      • Paraffin embedded HER2+ tumor (IDC31) sections incubated with ErbB2 (ab2428). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/100 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 5 min. (This antibody has also been tested successfully on xenograft derived from ER+ pleural effusion and the HER2+ amplified breast cancer cell line BT474).
      • Paraffin embedded primary ER+ breast tumor sections incubated with Progesterone Receptor (ab2764). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/100 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 5-10 min.
      • Paraffin embedded primary ER+ breast tumor sections incubated with Estrogen receptor alpha (ab37438). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/50 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 5 min. (This antibody has also been tested successfully on xenograft derived from ER+ pleural effusion).
      • Paraffin embedded primary ER- breast tumor sections incubated with p53 (ab26). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/50 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 10 min.
      • Paraffin embedded primary ER- breast tumor sections incubated with ki67 (ab15580). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/100 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 2-3 min.

      References for Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR) (ab104827)

      This product has been referenced in:
      • Ucar A  et al. miR-212 and miR-132 are required for epithelial stromal interactions necessary for mouse mammary gland development. Nat Genet 42:1101-8 (2010). Read more (PubMed: 21057503) »
      • Abraira VE  et al. Vertebrate Lrig3-ErbB interactions occur in vitro but are unlikely to play a role in Lrig3-dependent inner ear morphogenesis. PLoS One 5:e8981 (2010). Read more (PubMed: 20126551) »

      See all 5 Publications for this product

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