The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
Use a concentration of 1 - 5 µg/ml.
FunctionMay play a role in tumorigenesis as a tumor suppressor. Altered expression of this protein and disruption of the molecular pathway it is involved in, may contribute directly to or modify tumorigenesis.
Tissue specificityExpressed at low level in many tissues. Not expressed in 80% of tumor cell lines tested.
Sequence similaritiesContains 1 VIT domain. Contains 1 VWFA domain.
Loss of heterozygosity 11 chromosomal region 2 gene A antibody
Loss of heterozygosity 11 chromosomal region 2 gene A protein antibody
Ortholog of mouse AW551984 antibody
von Willebrand factor A domain containing 5A antibody
von Willebrand factor A domain-containing protein 5A antibody
Anti-Breast cancer suppressor candidate 1 antibody images
Western blot - Breast cancer suppressor candidate 1 antibody (ab64977)
All lanes : Anti-Breast cancer suppressor candidate 1 antibody (ab64977) at 1/500 dilution
Lane 1 : extracts from 293 cells (5-30µg total protein) Lane 2 : extracts from 293 cells (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size : 86 kDa Observed band size : 86 kDa Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence-Breast cancer suppressor candidate 1 antibody(ab64977)
ICC/IF image of ab64977 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64977, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Breast cancer suppressor candidate 1 antibody (ab64977)
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