Breast (Human) Tissue Lysate - adult normal tissue (ab42045)

Overview

  • Product nameBreast (Human) Tissue Lysate - adult normal tissue
    See all Breast lysates
  • General notesDiagnosis: Normal Sex: Female Age: 75 years Soluble protein fraction

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: 0.25% Sodium Deoxycholate, 0.1% SDS, PBS, 1µg/ml Pepstatin A, 1µg/ml Leupeptin, 1µg/ml Aprotinin, 1mM PMSF, 1mM EDTA, 1mM Sodium fluoride, 1mM Sodium Orthovanadate, pH 7.4
    Please note that 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate; as a result the lysate may not be suitable for applications which require native protein in a non-denaturing buffer. The final Laemmli buffer concs are: 31.25mM Tris HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.005% Bromophenol Blue, 25mM TCEP.
  • Concentration information loading...
  • Purification NotesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Lysate notes This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab42045: Extraction 1: soluble protein fraction ab42046 Human Breast Tumor Tissue lysate ab42045 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43488 Human Breast Tumor Tissue lysate ab43487 Human Breast Normal Tissue lysate (matched)
  • Research Areas

    References for Breast (Human) Tissue Lysate - adult normal tissue (ab42045)

    ab42045 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More

    I am sorry that lysates are not working for you. I have following suggestions that could help in determining why it is more difficult to compare the product of housekeeping genes, between tissue lysates and cells lysates

    1. Many researchers ...

    Read More

    Thank you for your enquiry regarding ab43338, ab43343 and ab42045 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for supplying ...

    Read More

    According to our records, ab42045, ab43338 and ab433343was proving difficult to use inWB and we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaiting more details in order to help us...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"