Overview

  • Product nameBrefeldin A
  • Description
    Inhibitor of ADP-ribosylation factor
  • Alternative names
    • Ascotoxin
    • BFA
    • Cyanein
    • Decumbin
  • Biological descriptionReversible inhibitor of protein translocation from the endoplasmic reticulum to the Golgi apparatus. Inhibits binding of the cytosolic coat protein, β-COP and ADP-ribosylation factor (ARF) to Golgi membranes and inhibits GDP-GTP exchange.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120299 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Brefeldin A images

    • ab84340 staining golgin-97 in MCF7 cells treated with brefeldin A (ab120299), by ICC/IF. Increase in Golgin-97 expression correlates with increased concentration of brefeldin A, as described in literature.
      The cells were incubated at 37°C for 1.5 h in media containing different concentrations of ab120299 (brefeldin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab84340 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Brefeldin A (ab120299)

    This product has been referenced in:
    • Lippincott-Schwartz J  et al. Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic. Cell 67:601-16 (1991). Read more (PubMed: 1682055) »
    • Donaldson JG  et al. Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatus. J Cell Biol 112:579-88 (1991). Read more (PubMed: 1993732) »
    • Pelham HR Multiple targets for brefeldin A. Cell 67:449-51 (1991). Read more (PubMed: 1934055) »

    See 1 Publication for this product

    Product Wall

    We would always recommend storing the reconstituted product at -20ºC until it is ready for use as other suppliers also suggest. Generally, these aliquots will be useable for up to one month. Some customers still use the product for up to 3 months ...

    Read More

    Thank you for contacting us.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will off...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"