The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay was 1:62500.
Use a concentration of 1.25 µg/ml. Predicted molecular weight: 74 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionGeneral activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
Sequence similaritiesBelongs to the TFIIB family. Contains 1 TFIIB-type zinc finger.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.