Overview

  • Product nameAnti-BRF1 antibody
    See all BRF1 primary antibodies
  • Description
    Rabbit polyclonal to BRF1
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, mapping to a region between amino acids 550-600 of Human BRF1 (NP_001510.2)

  • Positive control
    • HeLa or 293T whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab74221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionGeneral activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
  • Sequence similaritiesBelongs to the TFIIB family.
    Contains 1 TFIIB-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • B related factor 1 antibody
    • B-related factor 1 antibody
    • BRF antibody
    • BRF-1 antibody
    • brf1 antibody
    • BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) antibody
    • General transcription factor IIIB, 90kD subunit antibody
    • GTF3B antibody
    • hBRF antibody
    • hTFIIIB90 antibody
    • RNA polymerase III antibody
    • subunit 2 antibody
    • TAF3B2 antibody
    • TAF3C antibody
    • TAFIII90 antibody
    • TATA box binding protein (TBP) associated factor 3C antibody
    • TATA box binding protein (TBP) associated factor, RNA polymerase III, GTF3B subunit 2 antibody
    • TATA box-binding protein-associated factor antibody
    • TBP associated factor, RNA polymerase III, 90kD antibody
    • TF3B_HUMAN antibody
    • TF3B90 antibody
    • TFIIIB90 antibody
    • Transcription factor IIIB 90 kDa subunit antibody
    • Transcription initiation factor IIIB antibody
    see all

Anti-BRF1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRF1 with ab74221 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-BRF1 antibody (ab74221) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 140 kDa,200 kDa. We are unsure as to the identity of these extra bands.
  • HeLa whole cell lysate (1mg) was subjected to immunoprecipitation using ab74221 (3µg/mg lysate; lane 1) or a control IgG antibody (lane 2). 20% of the immunoprecipitates were used for Western blotting and bands were identified using ab74221 at a concentration of 1µg/ml. A band at the predicted molecular weight of 74kDa was detected using chemiluminescence with an exposure time of 3 seconds.

References for Anti-BRF1 antibody (ab74221)

This product has been referenced in:

See 1 Publication for this product

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