Overview

  • Product name
  • Description
    Rabbit polyclonal to BRF2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 2-235 of Human BRF2 (Uniprot ID: Q9HAW0).

  • Positive control
    • A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells; Human Ca922 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-BRF2 antibody (ab154658) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling BRF2 with ab154658 at 1/200 dilution. Lower image is co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human Ca922 xenograft tissue labeling BRF2 with ab154658 at 1/500 dilution.

References

ab154658 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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