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Goat polyclonal to BRF2
Synthetic peptide: QAARQAATSVPNPP, corresponding to C terminal amino acids 406-419 of Human BRF2.
HeLa cell lysate. Human placenta lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 20418882
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).Can be blocked with
Human BRF2 peptide (ab23297).
General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
Information by UniProt
B-related factor 2 antibody
Anti-BRF2 antibody images
Western blot - BRF2 antibody (ab17011)
Predicted band size : 48 kDa ab17011 staining (1.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ChIP - BRF2 antibody (ab17011) Image courtesy of Dr Andrew Oler by Abreview.
ab17011 used in ChIP. For details please see Abreview.
References for Anti-BRF2 antibody (ab17011)
This product has been referenced in:
et al. Integrative genomic analyses identify BRF2 as a novel lineage-specific oncogene in lung squamous cell carcinoma. PLoS Med 7:e1000315 (2010).
Read more (PubMed: 20668658) »
et al. Human RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors. Nat Struct Mol Biol 17:620-8 (2010).
Read more (PubMed: 20418882) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"