Anti-BRF2 antibody (ab17011)
Key features and details
- Goat polyclonal to BRF2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRF2 antibody
See all BRF2 primary antibodies -
Description
Goat polyclonal to BRF2 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide: QAARQAATSVPNPP, corresponding to C terminal amino acids 406-419 of Human BRF2.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. -
Sequence similarities
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55290 Human
- Omim: 607013 Human
- SwissProt: Q9HAW0 Human
- Unigene: 726335 Human
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Alternative names
- B-related factor 2 antibody
- BRF-2 antibody
- BRF2 antibody
see all
Images
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ab17011 at 1.5
µ g/ml detecting BRF2 from HeLa cell lysate (35ug total protein per lane) by Western blot. Primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (3)
ab17011 has been referenced in 3 publications.
- Gouge J et al. Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2. Cell 163:1375-87 (2015). PubMed: 26638071
- Lockwood WW et al. Integrative genomic analyses identify BRF2 as a novel lineage-specific oncogene in lung squamous cell carcinoma. PLoS Med 7:e1000315 (2010). WB ; Human . PubMed: 20668658
- Oler AJ et al. Human RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors. Nat Struct Mol Biol 17:620-8 (2010). IP ; Human . PubMed: 20418882