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Synthetic peptide corresponding to a region between residue 75 and 125 of human BRG1
Our Abpromise guarantee covers the use of ab70558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|ChIP||Use at an assay dependent concentration.|
|WB||1/2000 - 1/10000. Detects a band of approximately 185 kDa (predicted molecular weight: 185 kDa).|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/250 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|