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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Mouse BRG1 aa 200-300.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager.
Alternative versions available: Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487. This product is a recombinant rabbit monoclonal antibody.
Anti-BRG1 antibody (Alexa Fluor® 488) [EPNCIR111A] (ab196314)
Anti-BRG1 antibody (Alexa Fluor® 647) [EPNCIR111A] (ab196535)
Anti-BRG1 antibody (HRP) [EPNCIR111A] (ab196315)
Anti-BRG1 antibody (Alexa Fluor® 594) [EPNCIR111A] (ab207052)
Anti-BRG1 antibody (Alexa Fluor® 568) [EPNCIR111A] (ab207055)
Alternative versions available:
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab110641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 185 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Antigen retrieval is recommended. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.|
|ICC/IF||1/100 - 1/250.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: BRG1 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: Molt-4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab110641 observed at 185 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab110641 was shown to specifically react with BRG1 when BRG1 knockout samples were used. Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. ab110641 and ab18058 (loading control to Vinculin) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ChIP analysis using ab110641 binding BRG1 in mouse bone marrow derived macrophages. Cells were cross-linked for 10 minutes with formaldehyde. Samples were incubated with undiluted primary antibody for 16 hours at 4°C. Protein binding was detected using real-time PCR.
Positive control: PU.1 antibody.
Negative Control: rabbit IgG.