Our Abpromise guarantee covers the use of ab110641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Predicted molecular weight: 185 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionTranscriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
    • Tissue specificityColocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).
    • Involvement in diseaseDefects in SMARCA4 are the cause of rhabdoid tumor predisposition syndrome type 2 (RTPS2) [MIM:613325]. RTPS2 is a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood.
    • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
      Contains 1 bromo domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HSA domain.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent helicase SMARCA4 antibody
      • ATP-dependent helicase SMARCA4 antibody
      • BAF 190 antibody
      • BAF190 antibody
      • BAF190A antibody
      • Brahma protein like 1 antibody
      • BRG1 antibody
      • BRG1 associated factor 190A antibody
      • BRG1 protein antibody
      • BRG1-associated factor 190A antibody
      • BRM/SWI2 related gene 1 antibody
      • Global transcription activator homologous sequence antibody
      • global transcription activator snf2l4 antibody
      • Homeotic gene regulator antibody
      • hSNF2b antibody
      • Mitotic growth and transcription activator antibody
      • MRD16 antibody
      • Nuclear protein GRB1 antibody
      • Protein brahma homolog 1 antibody
      • Protein BRG-1 antibody
      • Protein BRG1 antibody
      • RTPS2 antibody
      • SMARC A4 antibody
      • SMARCA4 antibody
      • SMCA4_HUMAN antibody
      • SNF2 antibody
      • SNF2 beta antibody
      • SNF2 like 4 antibody
      • SNF2-beta antibody
      • SNF2B antibody
      • SNF2L4 antibody
      • SNF2LB antibody
      • Sucrose nonfermenting like 4 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 antibody
      • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 antibody
      • SWI2 antibody
      • Transcription activator BRG1 antibody
      see all

    Anti-BRG1 antibody [EPNCIR111A] images

    • Predicted band size : 185 kDa
      Additional bands at : 185 kDa. We are unsure as to the identity of these extra bands.

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: BRG1 knockout HAP1 cell lysate (20 µg)
      Lane 3: K562 cell lysate (20 µg)
      Lane 4: Molt-4 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab110641 observed at 185 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab110641 was shown to specifically react with BRG1 when BRG1 knockout samples were used. Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. ab110641 and ab18058 (loading control to Vinculin) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10 000 dilution for 1 hour at room temperature before imaging.

    • ab110641 at 1/100 dilution staining BRG1 in Human testis tissue by Immunohistochemistry, Paraffin-embedded tissue.
    • ab110641 at 1/100 dilution staining BRG1 in HeLa cells by Immunofluorescence.
    • All lanes : Anti-BRG1 antibody [EPNCIR111A] (ab110641) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MOLT4 cell lysate
      Lane 4 : NIH3T3 cell lysate
      Lane 5 : PC12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 185 kDa
    • ab110641 at 1/100 dilution staining BRG1 in Human kidney tissue by Immunohistochemistry, Paraffin-embedded tissue.
    • ChIP analysis using ab110641 binding BRG1 in mouse bone marrow derived macrophages. Cells were cross-linked for 10 minutes with formaldehyde. Samples were incubated with undiluted primary antibody for 16 hours at 4°C. Protein binding was detected using real-time PCR.
      Positive control: PU.1 antibody.
      Negative Control: rabbit IgG.

      See Abreview

    References for Anti-BRG1 antibody [EPNCIR111A] (ab110641)

    This product has been referenced in:
    • Agaimy A  et al. Loss of expression of the SWI/SNF complex is a frequent event in undifferentiated/dedifferentiated urothelial carcinoma of the urinary tract. Virchows Arch 469:321-30 (2016). Read more (PubMed: 27339451) »
    • Coatham M  et al. Concurrent ARID1A and ARID1B inactivation in endometrial and ovarian dedifferentiated carcinomas. Mod Pathol N/A:N/A (2016). Read more (PubMed: 27562491) »

    See all 21 Publications for this product

    Product Wall

    Application Western blot
    Sample Zebrafish Cell lysate - other (fibroblast cell tipe)
    Gel Running Conditions Reduced Denaturing (SDS-PAGE 7.5%)
    Loading amount 100 µg
    Specification fibroblast cell tipe
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mr. Jorge Castillo

    Verified customer

    Submitted Mar 28 2016

    Application Western blot
    Sample Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions Non-reduced Denaturing
    Loading amount 50 µg
    Specification Brain
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Jun 05 2015

    Application ChIP
    Detection step Real-time PCR
    Sample Mouse Cell lysate - whole cell (bone marrow derived macrophages)
    Specification bone marrow derived macrophages
    Negative control rabbit IgG
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Positive control PU.1 antibody

    Dr. Silvia Bonifacio

    Verified customer

    Submitted May 02 2014

    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Human Cell lysate - nuclear (Human Cancer Cell line)
    Specification Human Cancer Cell line
    Total protein in input 500 µg

    Abcam user community

    Verified customer

    Submitted Apr 24 2014

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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    I am sorry hear about the disappointing results. If customer have purchased the secondary antibody from Abcam (ab6718) and if the problem is due to secondary antibody then I am happy to replace it for this customer.
    Please let me know the order num...

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    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
    I have read the details you have kindly provided and have following further questions for better understanding of the problem;
    - What was ...

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    Thank you for your enquiry.

    At this time we have no data regarding the use of ab110641 in frozen sections. It certainly may be useful in this application, as many antibodies that are useful in IHC-P can also be used in frozen sections.

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    Thank you for providing details.
    This is strange, if the red cells are seen even without primary antibody then the problem is either due to secondary antibody or due to protocol. I have checked the protocol and I find no problem with it other than ...

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