• Product nameAnti-BRIT1 antibody
    See all BRIT1 primary antibodies
  • Description
    Rabbit polyclonal to BRIT1
  • Tested applicationsSuitable for: IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 555-701 of Human BRIT1 (Q8NEM0).

  • Positive control
    • Human cerebral cortex RT-4 cell lysate U-251 MG cell lysate



Our Abpromise guarantee covers the use of ab121277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionImplicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.
  • Tissue specificityExpressed in fetal brain, liver and kidney.
  • Involvement in diseaseDefects in MCPH1 are the cause of microcephaly primary type 1 (MCPH1) [MIM:251200]; also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait.
  • Sequence similaritiesContains 3 BRCT domains.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRCT repeat inhibitor of TERT expression 1 antibody
    • BRIT 1 antibody
    • FLJ12847 antibody
    • Hypothetical protein FLJ12847 antibody
    • MCPH 1 antibody
    • MCPH1 antibody
    • MCPH1_HUMAN antibody
    • MCT antibody
    • Microcephalin 1 antibody
    • Microcephalin antibody
    • Microcephaly primary autosomal recessive 1 antibody
    see all

Anti-BRIT1 antibody images

  • All lanes : Anti-BRIT1 antibody (ab121277) at 1/250 dilution

    Lane 1 : Markers (kDa)
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human plasma
    Lane 5 : Liver tissue lysate
    Lane 6 : Tonsil tissue lysate

    HPR conjugated anti-rabbit antibody at 1/3000 dilution
    Developed using the ECL technique
  • ab121277, at 1/75, staining BRIT1 in neuronal cells of paraffin embedded Human cerebral cortex by Immunohistochemistry.
  • Immunohistochemical staining of Human testis shows strong positivity in a subset of cells in seminiferus ducts using ab121277

References for Anti-BRIT1 antibody (ab121277)

This product has been referenced in:
  • Ge C  et al. The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex. PLoS One 10:e0144957 (2015). Read more (PubMed: 26683461) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (U2OS and RPE)
Total protein in input 3.5 µg
Specification U2OS and RPE
Immuno-precipitation step Protein A

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Submitted Apr 16 2012