Anti-MCPH1 antibody (ab121277)
Key features and details
- Rabbit polyclonal to MCPH1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MCPH1 antibody -
Description
Rabbit polyclonal to MCPH1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MCPH1 aa 555-701.
Database link: Q8NEM0 -
Positive control
- IHC-P: Human cerebral cortex, cerebellum and testis tissue sections. WB: NTERA-2 cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex. -
Tissue specificity
Expressed in fetal brain, liver and kidney. -
Involvement in disease
Defects in MCPH1 are the cause of microcephaly primary type 1 (MCPH1) [MIM:251200]; also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait. -
Sequence similarities
Contains 3 BRCT domains. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
-
Database links
- Entrez Gene: 79648 Human
- Omim: 607117 Human
- SwissProt: Q8NEM0 Human
- Unigene: 656769 Human
- Unigene: 708770 Human
- Unigene: 721952 Human
-
Alternative names
- BRCT repeat inhibitor of TERT expression 1 antibody
- BRIT 1 antibody
- FLJ12847 antibody
see all
Images
-
Anti-MCPH1 antibody (ab121277) at 0.4 µg/ml + NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) cell lysate
-
Immunohistochemical analysis of paraffin embedded human testis tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded human cerebral cortex tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded human cerebellum tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded human liver tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab121277 has been referenced in 3 publications.
- Liu L et al. Silencing BRIT1 Facilitates the Abilities of Invasiveness and Migration in Trophoblast Cells. Med Sci Monit 24:7451-7458 (2018). PubMed: 30337515
- Ge C et al. The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex. PLoS One 10:e0144957 (2015). PubMed: 26683461
- Ge C et al. BRUCE regulates DNA double-strand break response by promoting USP8 deubiquitination of BRIT1. Proc Natl Acad Sci U S A 112:E1210-9 (2015). PubMed: 25733871