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Our Abpromise guarantee covers the use of ab2612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 93 kDa).|
LCL1, LCL2 and LCL3 are three patient derived fibroblast cell lines.
The indicated dilution of antibody was incubated with 30
The middle band is BRIT1 (this has been confirmed by siRNA analysis).
15 second exposure.LCL1, LCL2, and LCL3 are three patient derived fibroblast cell lines. The middle band is BRIT1 (denoted by arrow) as determined by treatment with siRNA.
Image courtesy of Human Protein Atlas
ab2612 staining in human lung, showing staining of the surface epithelial cells (in brown). Paraffin embedded lung tissue was incubated with ab2612 (1:100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab2612 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Immunohistochemistical detection of BRIT1 using antibody (ab2612) on paraformaldehyde perfusion fixed mouse heart tissue sections. Primary antibody diluted @ 1/300 & incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit conjugated to biotin (1/300). The antibody produced a weak cytoplasmic staining in cardiac cells.
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