Overview

  • Product nameAnti-Brk antibody
    See all Brk primary antibodies
  • Description
    Rabbit polyclonal to Brk
  • Specificityab65221 detects endogenous levels of total Brk protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from N terminus of human Brk

  • Positive control
    • LOVO cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab65221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

  • FunctionPhosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
  • Tissue specificityEpithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.
  • Cellular localizationCytoplasm. Nucleus. Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.
  • Information by UniProt
  • Database links
  • Alternative names
    • Breast tumor kinase antibody
    • Breast tumour kinase antibody
    • Protein tyrosine kinase 6 antibody
    • Protein-tyrosine kinase 6 antibody
    • Ptk6 antibody
    • PTK6_HUMAN antibody
    • Tyrosine protein kinase BRK antibody
    • Tyrosine-protein kinase BRK antibody
    see all

Anti-Brk antibody images

  • All lanes : Anti-Brk antibody (ab65221) at 1/500 dilution

    Lane 1 : LOVO cell extract
    Lane 2 : LOVO cell extract with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa

References for Anti-Brk antibody (ab65221)

ab65221 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"