• Product nameAnti-BRMS1 antibody
    See all BRMS1 primary antibodies
  • Description
    Rabbit polyclonal to BRMS1
  • Specificityab65244 detects endogenous levels of total BRMS1 protein.
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from N terminal of human BRMS1.

  • Positive control
    • 293 cell extracts.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab65244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22076152
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).


  • FunctionTranscriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
  • Tissue specificityExpression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
  • Sequence similaritiesBelongs to the BRMS1 family.
  • Cellular localizationNucleus. Cytoplasm. Predominantly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • AV003220 antibody
    • AW554636 antibody
    • Breast cancer metastasis suppressor 1 antibody
    • Breast cancer metastasis-suppressor 1 antibody
    • BRMS1 antibody
    • BRMS1_HUMAN antibody
    • DKFZp564A063 antibody
    • MGC95128 antibody
    see all

Anti-BRMS1 antibody images

  • All lanes : Anti-BRMS1 antibody (ab65244) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunising peptide

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
  • ab65244 staining BRMS1 in Human breast cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was blocked with 10% goat serum for 60 minutes; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in diluent) overnight at 4°C, incubated with HRP-conjugated secondary antibody, before being stained with DAB.

References for Anti-BRMS1 antibody (ab65244)

This product has been referenced in:
  • Al-Alwan M  et al. Fascin is a key regulator of breast cancer invasion that acts via the modification of metastasis-associated molecules. PLoS One 6:e27339 (2011). IHC-P ; Human . Read more (PubMed: 22076152) »

See 1 Publication for this product

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