The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22076152
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
All lanes : Anti-BRMS1 antibody (ab65244) at 1/500 dilution
Lane 1 : 293 cell extract Lane 2 : 293 cell extract with immunising peptide
Predicted band size : 28 kDa Observed band size : 28 kDa Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRMS1 antibody (ab65244)Image from Al-Alwan M et al., PLoS One. 2011;6(11):e27339. Epub 2011 Nov 4. Fig 4.; doi:10.1371/journal.pone.0027339; November 4, 2011, PLoS ONE 6(11): e27339.
ab65244 staining BRMS1 in Human breast cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was blocked with 10% goat serum for 60 minutes; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in diluent) overnight at 4°C, incubated with HRP-conjugated secondary antibody, before being stained with DAB.
Al-Alwan M et al. Fascin is a key regulator of breast cancer invasion that acts via the modification of metastasis-associated molecules. PLoS One6:e27339 (2011).
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