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Synthetic peptide derived from within residues 400 to the C-terminus of Mouse BRN3A.
(Peptide available as ab30867.)
Our Abpromise guarantee covers the use of ab30880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 0.54 µg/ml.|
Immuofluorescent staining for BRN3A in the rat dorsal root ganglion using Rabbit polyclonal to BRN3A (ab30880; images taken with X20 objective). ab30880 was used at 1/1000 (0.54µg/ml) incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.