Recombinant
RabMAb

Anti-BRN3A antibody [EP1972Y] (ab81213)

Overview

  • Product name
    Anti-BRN3A antibody [EP1972Y]
    See all BRN3A primary antibodies
  • Description
    Rabbit monoclonal [EP1972Y] to BRN3A
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the C terminal region of Human BRN3A

  • Positive control
    • WB: Human brain lysate; ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab81213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • Function
    Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
  • Tissue specificity
    Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.
  • Sequence similarities
    Belongs to the POU transcription factor family. Class-4 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Developmental stage
    Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain specific homeobox/POU domain protein 3A antibody
    • Brain-3A antibody
    • Brain-specific homeobox/POU domain protein 3A antibody
    • Brn 3.0 antibody
    • BRN 3A antibody
    • Brn-3A antibody
    • Brn3 antibody
    • BRN3A antibody
    • class 4 antibody
    • FLJ13449 antibody
    • Homeobox/POU domain protein RDC 1 antibody
    • Homeobox/POU domain protein RDC-1 antibody
    • Homeobox/POU domain protein RDC1 antibody
    • Oct T1 antibody
    • Oct-T1 antibody
    • OctT1 antibody
    • PO4F1_HUMAN antibody
    • POU class 4 homeobox 1 antibody
    • POU domain antibody
    • POU Domain Class 4 Transcription Factor 1 antibody
    • Pou4f1 antibody
    • RDC 1 antibody
    • RDC1 antibody
    • transcription factor 1 antibody
    see all

Images

  • Immunofluorescent staining of HeLa cells using 1/100 ab81213.
  • All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213)

    Lane 1 : LNCaP
    Lane 2 : SW480
    Lane 3 : PC-3
    Lane 4 : Human brain lysate


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) at 1/2000 dilution

    Lane 1 : Adult mouse cerebellum lysate
    Lane 2 : Adult mouse lower brainstem lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated Mouse anti-Rabbit IgG monoclonal. at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa


    Exposure time : 2 minutes

    This image is courtesy of an Anonymous Abreview.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab81213 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81213, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Çerman E  et al. Retinal Electrophysiological Effects of Intravitreal Bone Marrow Derived Mesenchymal Stem Cells in Streptozotocin Induced Diabetic Rats. PLoS One 11:e0156495 (2016). IF . Read more (PubMed: 27300133) »
  • Lee KS  et al. Human sensory neurons derived from induced pluripotent stem cells support varicella-zoster virus infection. PLoS One 7:e53010 (2012). Flow Cyt ; Human . Read more (PubMed: 23285249) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse eye)
Permeabilization
Yes - 0.3% triton X-100
Specification
mouse eye
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 18 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Retinal ganglion cells)
Permeabilization
Yes - Triton-X-100
Specification
Retinal ganglion cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Dr. Kris Pike

Verified customer

Submitted Aug 11 2015

With the frozen sections, did you do a fix and permeabilize step. I didnt notice that in your protocol. If you haven't then that 's probably the reason for no staining. Its important to do that so that the epitope is accessible.
I would also just ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
Specification
Dorsal Root Ganglia (DRG) neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - tween 20
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (Brain explant)
Specification
Brain explant
Username

Abcam user community

Verified customer

Submitted Oct 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (cerebellum & lower portion of brainstem (adult))
Loading amount
20 µg
Specification
cerebellum & lower portion of brainstem (adult)
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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