Overview

  • Product nameAnti-BRP44 antibody
    See all BRP44 primary antibodies
  • Description
    Rabbit polyclonal to BRP44
  • Specificityab111380 detects endogenous levels of total BRP44 protein.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of Human BRP44.

  • Positive control
    • Human lung carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 0.88% Sodium chloride
    Note: without Mg2+ and Ca2+
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111380 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-BRP44 antibody images

  • ab111380 at 1/50 dilution staining BRP44 in paraffin-embedded Human Lung carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

References for Anti-BRP44 antibody (ab111380)

ab111380 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Leukemia cell lines)
Gel Running Conditions Reduced Denaturing (4-20% and 18%)
Loading amount 40 µg
Specification Leukemia cell lines
Blocking step Licor:PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

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Submitted Nov 04 2016

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (12)
Sample Human Cell lysate - whole cell (Lane 1 U2OS, Lane 2 MIN6)
Specification Lane 1 U2OS, Lane 2 MIN6
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Nov 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"