• Product nameAnti-BRP44L antibody
    See all BRP44L primary antibodies
  • Description
    Rabbit polyclonal to BRP44L
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human BRP44L (internal sequence).

  • Positive control
    • WB: extracts from COLO and HeLa cells IHC-P: human heart tissue ICC/IF: A549 cells


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab74871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 12 kDa.
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.


Anti-BRP44L antibody images

  • All lanes : Anti-BRP44L antibody (ab74871) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from HeLa cells
    Lane 3 : extracts from COLO cells with immunizing peptide at 10 µg

    Lysates/proteins at 20 µg per lane.

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • ab74871, at a 1/50 dilution, staining BRP44L in paraffin embedded human heart tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
  • ab74871, at a 1/500 dilution, staining BRP44L in A549 cells by Immunofluorescence in the absence (left image) or presence (right image) of the immunizing peptide.

References for Anti-BRP44L antibody (ab74871)

This product has been referenced in:
  • Martínez-Zamora A  et al. Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier. PLoS One 10:e0144273 (2015). WB ; Human . Read more (PubMed: 26642043) »
  • Rohatgi N  et al. Novel insulin sensitizer modulates nutrient sensing pathways and maintains ß-cell phenotype in human islets. PLoS One 8:e62012 (2013). ICC/IF ; Human . Read more (PubMed: 23650507) »

See all 2 Publications for this product

Product Wall

Expiration date: 16/08/2012

I am very pleased to hear you would like to accept our offer and testab74871 in Mouse/Rat in WB. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expira...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Retina)
Permeabilization No
Specification Retina
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde

Allison Grenell

Verified customer

Submitted Jun 20 2017

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

The details your customer has kindly provided...

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Thank you for the precision and sorry about the confusion.

I created a new code for the test of ab116316 in Mouse: ***** (expire date DD MM YYYY)

Please ignore the previous code that has been inactivated on our system.

Expiration date: DD MM YYYY

I am very pleased to hear you would like to accept our offer and test ab74871 in Mouse. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, pleas...

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I am very sorry that you are having difficulties with the on-line datasheet of this antibody.

The unit size is 100 µg and the price is £220.00 ($325.00). The product is in liquid format and it is shipped at 4°C. Upon delivery ...

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Thank you for your enquiry regarding ab74871.

I can confirm tha this antibody is still available and currently we have 5 vials in stock.

I hope this helps and if I can assist further, please do not hesitate to contact me.

Thank you for your interest.

Mouse and rat are predicted species so it is likely that ab74871 will recognize them but w do not have any experimental data to support it.

I could certainly offer a testing discount for your customer. W...

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