Anti-MPC1 antibody (ab74871)
Key features and details
- Rabbit polyclonal to MPC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MPC1 antibody -
Description
Rabbit polyclonal to MPC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human MPC1 (internal sequence).
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Positive control
- WB: extracts from COLO and HeLa cells IHC-P: human heart tissue ICC/IF: A549 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 12 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 12 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Mediates the uptake of pyruvate into mitochondria. -
Involvement in disease
Mitochondrial pyruvate carrier deficiency -
Sequence similarities
Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 51660 Human
- Entrez Gene: 55951 Mouse
- Entrez Gene: 171087 Rat
- Omim: 614738 Human
- SwissProt: Q9Y5U8 Human
- SwissProt: P63030 Mouse
- SwissProt: P63031 Rat
- Unigene: 172755 Human
see all -
Alternative names
- Apoptosis regulating basic protein antibody
- Brain protein 44 like protein antibody
- Brain protein 44-like protein antibody
see all
Images
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All lanes : Anti-MPC1 antibody (ab74871) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from HeLa cells
Lane 3 : extracts from COLO cells with immunizing peptide at 10 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 12 kDa
Observed band size: 12 kDa -
ab74871, at a 1/50 dilution, staining MPC1 in paraffin embedded human heart tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
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ab74871, at a 1/500 dilution, staining MPC1 in A549 cells by Immunofluorescence in the absence (left image) or presence (right image) of the immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab74871 has been referenced in 6 publications.
- Tian GA et al. MPC1 Deficiency Promotes CRC Liver Metastasis via Facilitating Nuclear Translocation of ß-Catenin. J Immunol Res 2020:8340329 (2020). PubMed: 32851100
- Corbet C et al. Interruption of lactate uptake by inhibiting mitochondrial pyruvate transport unravels direct antitumor and radiosensitizing effects. Nat Commun 9:1208 (2018). PubMed: 29572438
- Ma X et al. Function of mitochondrial pyruvate carriers in hepatocellular carcinoma patients. Oncol Lett 15:9110-9116 (2018). PubMed: 29805642
- Wang D et al. Hypoxia induces lactate secretion and glycolytic efflux by downregulating mitochondrial pyruvate carrier levels in human umbilical vein endothelial cells. Mol Med Rep 18:1710-1717 (2018). PubMed: 29845198
- Martínez-Zamora A et al. Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier. PLoS One 10:e0144273 (2015). WB ; Human . PubMed: 26642043
- Rohatgi N et al. Novel insulin sensitizer modulates nutrient sensing pathways and maintains ß-cell phenotype in human islets. PLoS One 8:e62012 (2013). ICC/IF ; Human . PubMed: 23650507