Overview

  • Product name
  • Description
    Rabbit polyclonal to BRRN1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-193 of Human BRRN1 (Q15003).

  • Positive control
    • HeLa whole cell lysate; HeLa cells; Human Gastric carcinoma tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab126936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • Tissue specificity
    Widely expressed at low level. Expressed in proliferating cells.
  • Sequence similarities
    Belongs to the CND2 (condensin subunit 2) family.
  • Post-translational
    modifications
    Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation.
  • Cellular localization
    Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Barren Drosophila Homolog of 1 antibody
    • Barren homolog protein 1 antibody
    • BRRN antibody
    • BRRN1 antibody
    • CAP H antibody
    • CAPH antibody
    • Chromosome associated protein H antibody
    • Chromosome-associated protein H antibody
    • CND2_HUMAN antibody
    • Condensin complex subunit 2 antibody
    • Condensin subunit 2 antibody
    • Condensin-2 complex subunit H2 antibody
    • Condesin antibody
    • HCAP H antibody
    • hCAP-H antibody
    • KIAA0074 antibody
    • mCAP H antibody
    • MGC4616 antibody
    • Ncaph antibody
    • Non SMC condensin I complex subunit H antibody
    • Non-SMC condensin I complex subunit H antibody
    • XCAP H homolog antibody
    • XCAP-H homolog antibody
    see all

Images

  • Anti-BRRN1 antibody (ab126936) at 1/500 dilution + HeLa cell lysate at 30 µg

    Predicted band size : 83 kDa
  • ab126936, at 1/500 dilution, staining BRRN1 in methanol-fixed HeLa cells by Immunofluorescence analysis. The lower image is co-stained with Hoechst 33342.
  • ab126936, at 1/500 dilution, staining BRRN1 in paraffin-embedded Human Gastric carcinoma tissue by Immunohistochemistry.

References

ab126936 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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