Overview

  • Product nameAnti-BRSK1 antibody - N-terminal
    See all BRSK1 primary antibodies
  • Description
    Rabbit polyclonal to BRSK1 - N-terminal
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human BRSK1 conjugated to KLH.

  • Positive control
    • Mouse liver tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab71528 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/60 - 1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 87 kDa).
ELISA 1/1000.

Target

  • FunctionRequired for the polarization of forebrain neurons which endows axons and dendrites with distinct properties, possibly by locally regulating phosphorylation of microtubule-associated proteins (By similarity). May be involved in the regulation of G2/M arrest in response to UV- or methyl methane sulfonate (MMS)-induced, but not IR-induced, DNA damage. Phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo.
  • Tissue specificityWidely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Cellular localizationCytoplasm. Nucleus. Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • BR serine/threonine kinase 1 antibody
    • BR serine/threonine protein kinase 1 antibody
    • BR serine/threonine-protein kinase 1 antibody
    • Brain selective kinase 1 antibody
    • Brain-specific serine/threonine-protein kinase 1 antibody
    • BRSK 1 antibody
    • Brsk1 antibody
    • BRSK1_HUMAN antibody
    • FLJ43009 antibody
    • hSAD1 antibody
    • KIAA1811 antibody
    • Protein kinase SAD1A antibody
    • SAD 1 antibody
    • SAD1 antibody
    • SAD1 homolog antibody
    • SAD1 kinase antibody
    • SAD1A antibody
    • SADB antibody
    • SadB kinase short isoform antibody
    • Serine/threonine kinase SAD B antibody
    • Serine/threonine-protein kinase SAD-B antibody
    • Synapses of Amphids Defective homolog antibody
    see all

Anti-BRSK1 antibody - N-terminal images

  • Anti-BRSK1 antibody - N-terminal (ab71528) at 1/60 dilution + mouse liver tissue lysate at 35 µg

    Predicted band size : 87 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa,65 kDa. We are unsure as to the identity of these extra bands.

References for Anti-BRSK1 antibody - N-terminal (ab71528)

This product has been referenced in:
  • Eklund G  et al. The nuclear localization of ?-tubulin is regulated by SadB-mediated phosphorylation. J Biol Chem 289:21360-73 (2014). WB ; Human . Read more (PubMed: 24942739) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"