The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/60 - 1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 87 kDa).
FunctionRequired for the polarization of forebrain neurons which endows axons and dendrites with distinct properties, possibly by locally regulating phosphorylation of microtubule-associated proteins (By similarity). May be involved in the regulation of G2/M arrest in response to UV- or methyl methane sulfonate (MMS)-induced, but not IR-induced, DNA damage. Phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo.
Tissue specificityWidely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily. Contains 1 protein kinase domain. Contains 1 UBA domain.
Cellular localizationCytoplasm. Nucleus. Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage.