Overview

  • Product nameAnti-BRUNOL4 antibody
    See all BRUNOL4 primary antibodies
  • Description
    Rabbit polyclonal to BRUNOL4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide from within internal sequence amino acids 288-337: MAAFAAAQMQ QMAALNMNGL AAAPMTPTSG GSTPPGITAP AVPSIPSPIG, of Human BRUNOL4 (NP_064565).

  • Positive control
    • OVCAR3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceBRUNOL4 is an RNA binding protein implicated in the regulation of pre mRNA alternative splicing. It mediates exon inclusion and/or exclusion in pre mRNA that are subject to tissue specific and developmentally regulated alternative splicing. It specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition; promotes exclusion of both the smooth muscle (SM) and non muscle (NM) exons in actinin pre mRNAs; binds to muscle specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre mRNA.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • bruno like 4, RNA binding protein antibody
    • Bruno like 4, RNA binding protein (Drosophila) antibody
    • Bruno like protein 4 antibody
    • BRUNOL 4 antibody
    • CELF 4 antibody
    • CELF4 antibody
    • CUG BP and ETR 3 like factor 4 antibody
    • CUGBP, Elav like family member 4 antibody
    • LYST interacting protein LIP9 antibody
    • RNA binding protein BRUNOL 4 antibody
    • RNA binding protein BRUNOL4 antibody
    see all

Anti-BRUNOL4 antibody images

  • Anti-BRUNOL4 antibody (ab94645) at 1 µg/ml (in 5% skim milk / PBS buffer) + OVCAR3 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa

References for Anti-BRUNOL4 antibody (ab94645)

ab94645 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control Protein A dynabeads without antibody bound
Immuno-precipitation step Other - Protein A dynabeads
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Submitted Mar 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"