Overview

  • Product nameAnti-BS69 antibody
    See all BS69 primary antibodies
  • Description
    Rabbit polyclonal to BS69
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: HPMYRRLVHS AVDVPTIQEK VNEGKYRSYE EFKADAQLLL HNTVIFYGAD, corresponding to N terminal amino acids 145-194 of Human BS69

  • Positive control
    • WB: HepG2 cell lysate. IHC-P: human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab56025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevanceBS69 is a factor previously identified as an adenovirus E1A-associated protein. The protein localizes to the nucleus. It functions as a transcriptional repressor, and expression of E1A inhibits this repression. Two transcript variants encoding different isoforms have been found for this gene.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Adenovirus 5 E1A binding protein antibody
    • Bone morphogenetic protein receptor associated molecule 1 antibody
    • BRAM1 antibody
    • BS69 variant 1 antibody
    • BS69 variant 2 antibody
    • BS69 variant 3 antibody
    • BS69 variant 4 antibody
    • RP11-486H9.1 antibody
    • Zinc finger MYND domain containing 11 antibody
    • zinc finger MYND domain containing protein 11 antibody
    • zinc finger, MYND type containing 11 antibody
    • ZMYND11 antibody
    see all

Anti-BS69 antibody images

  • Anti-BS69 antibody (ab56025) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling BS69 with ab56025 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-BS69 antibody (ab56025)

ab56025 has not yet been referenced specifically in any publications.

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The predicted molecular weight of BS69 is 66KDa. I will ask the laboratory to retest the western blot and let you know the results as soon as possible.

Thank you for your patience and cooperation ...

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