Overview

  • Product name
    Anti-BSD antibody
  • Description
    Rabbit polyclonal to BSD
  • Specificity
    The antibody detects a 13 kDa protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    not applicable
  • Immunogen

    Synthetic peptide conjugated to KLH corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase from Aspergillus Terrus.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was affinity purified using the N-terminal Bsd peptide (without carrier).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 13 kDa.
ELISA 1/4000.
ICC/IF 1/100.

Target

  • Relevance
    Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week.
  • Alternative names
    • Blasticidin-S deaminase antibody
    • BSD antibody

Images

  • All lanes : Anti-BSD antibody (ab38307)

    Lane 1 : 293 cells mock transfected
    Lane 2 : 293 cells transiently transfected with pLenti6/TR lentiviral vector
    Lane 3 : 293 cells transiently transfected with pLenti6/TR lentiviral vector


    Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    Each lane was loaded with 10-20µg total protein.
  • ab38307, staining BSD in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were fixed in paraformaldehyde (3.75%) for 30 min., then permeabilized in 0.2% Triton X-100 for 15 min. Blocking was done using 1% BSA for 30 min. The primary was used at 1/100 and then an appropriate secondary antibody conjugated to Texas Red was used.

References

This product has been referenced in:
  • Jammart B  et al. Very-low-density lipoprotein (VLDL)-producing and hepatitis C virus-replicating HepG2 cells secrete no more lipoviroparticles than VLDL-deficient Huh7.5 cells. J Virol 87:5065-80 (2013). Read more (PubMed: 23427158) »
  • de Wispelaere M & Yang PL Mutagenesis of the DI/DIII linker in dengue virus envelope protein impairs viral particle assembly. J Virol 86:7072-83 (2012). Read more (PubMed: 22532681) »

See all 2 Publications for this product

Customer reviews and Q&As

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Fibroblasts transduced with BSD-resistance gene)
Specification
Fibroblasts transduced with BSD-resistance gene
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citrate (pH 6.)
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Oct 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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