The antibody detects a 13 kDa protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 13 kDa.
Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week.
Blasticidin-S deaminase antibody
Western blot - Anti-BSD antibody (ab38307)
All lanes : Anti-BSD antibody (ab38307)
Lane 1 : 293 cells mock transfected Lane 2 : 293 cells transiently transfected with pLenti6/TR lentiviral vector Lane 3 : 293 cells transiently transfected with pLenti6/TR lentiviral vector
Immunocytochemistry/ Immunofluorescence - Anti-BSD antibody (ab38307)Image courtesy of Charles Mashburn and Dr. George Smith at the University of Kentucky, Spinal Cord and Brain Injury Research Center
ab38307, staining BSD in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were fixed in paraformaldehyde (3.75%) for 30 min., then permeabilized in 0.2% Triton X-100 for 15 min. Blocking was done using 1% BSA for 30 min. The primary was used at 1/100 and then an appropriate secondary antibody conjugated to Texas Red was used.
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