Overview

  • Product name
  • Description
    Mouse polyclonal to BST2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human BST2 protein (NP_004326.1).

  • Positive control
    • Human spleen tissue lysate, Jurkat cell lysate and BST2 transfected 293T cell lysate Human kidney tissue HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab88523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis.
  • Tissue specificity
    Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level).
  • Sequence similarities
    Belongs to the tetherin family.
  • Domain
    The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading.
  • Post-translational
    modifications
    Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.
  • Cellular localization
    Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bone marrow stromal antigen 2 antibody
    • Bone marrow stromal cell antigen 2 antibody
    • Bone marrow stromal cell antigen antibody
    • BST 2 antibody
    • BST-2 antibody
    • BST2 antibody
    • BST2_HUMAN antibody
    • CD 317 antibody
    • CD317 antibody
    • CD317 antigen antibody
    • HM1.24 antigen antibody
    • NPC A 7 antibody
    • Tetherin antibody
    see all

Images

  • Anti-BST2 antibody (ab88523) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size : 20 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Anti-BST2 antibody (ab88523) at 1 µg/ml + jurkat cell lysate at 50 µg

    Predicted band size : 20 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab88523, at 3 µg/ml, staining BST2 in formalin fixed, paraffin embedded human kidney tissue sections by Immunoperoxidase/Immunohistochemistry.
  • ab88523 at 10 µg/ml staining BST2 in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.

References

This product has been referenced in:
  • Jin X  et al. TXNIP mediates the differential responses of A549 cells to sodium butyrate and sodium 4-phenylbutyrate treatment. Cancer Med : (2016). Read more (PubMed: 28033672) »
  • Zou J  et al. The viral restriction factor tetherin prevents leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) from association with beclin 1 and B-cell CLL/lymphoma 2 (Bcl-2) and enhances autophagy and mitophagy. J Biol Chem 290:7269-79 (2015). Read more (PubMed: 25631043) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry.

Unfortunately we are not aware of any publication using the ab88523. This does not mean that this antibody has not been used in a publication, it just means that we do not know about it.

I can however co...

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Thank you for your reply.
I'm sending a free of charge vial of ab14694 on the order ***, which should arrive on Monday. Please keep me updated about the performance of this replacement antibody.
I look forward to hearing from you. Please let ...

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Thank you for your reply and for providing so many details about the protocol. This information will be helpful for our investigation of this antibody.
I've found an order going to ***. Can you confirm that this is your order? I can send the repla...

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According to our records, ab88523 was proving difficult to use in Western blot and we were in contact in order to help resolve the issue.
Looking at our correspondence, it appears that we are awaiting more details in order to help us better unders...

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Thank you for contacting us and letting us know about the trouble with this antibody.
I do have a couple of additional questions, so that I can better understand the situation:
1) Do you know your order number or lot number?
2) How has t...

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