Overview

  • Product nameAnti-BTBD15 antibody
    See all BTBD15 primary antibodies
  • Description
    Rabbit polyclonal to BTBD15
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 350-400 of Human BTBD15 according to BAI46608.1.

  • Positive control
    • Whole cell lysate from Jurkat cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab117814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    Anti-BTBD15 antibody images

    • Detection of BTBD15 by Western Blot of Immunprecipitate.
      An antibody to BTBD15 at 1µg/ml staining BTBD15 in Jurkat whole cell lysate following immunoprecipitation.
      Lane 1) IP using ab117814 at 6µg/mg lysate
      Lane 2) IP using control IgG.
      1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised chemiluminescence with a 10 second exposure.

    References for Anti-BTBD15 antibody (ab117814)

    ab117814 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 90 µg
    Gel Running Conditions Reduced Denaturing (6-18% gradient gel)
    Sample Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
    Specification Human osteosarcoma U2OS cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Jul 29 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"