Anti-BTB/POZ domain containing protein 6 antibody (ab66788)

Overview

  • Product nameAnti-BTB/POZ domain containing protein 6 antibody
    See all BTB/POZ domain containing protein 6 primary antibodies
  • Description
    Rabbit polyclonal to BTB/POZ domain containing protein 6
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Cat
  • Immunogen

    A region within synthetic peptide: MFNNELMADV HFVVGPPGAT RTVPAHKYVL AVGSSVFYAM FYGDLAEVKS, corresponding to N terminal amino acids 1-50 of Human BTB/POZ domain containing protein 6

  • Positive control
    • Wb: HepG2 cell lysate IHC-P: Human intestinal tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

  • FunctionAdapter protein for the CUL3 E3 ubiquitin-protein ligase complex. Involved in late neuronal development and muscle formation.
  • Tissue specificityExpressed in lens.
  • Sequence similaritiesContains 1 BTB (POZ) domain.
  • Cellular localizationCytoplasm > P-body. Cytoplasmic bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF1 coactivator 1| antibody
    • BDPL antibody
    • BTB (POZ) domain containing 6 antibody
    • BTB domain protein BDPL antibody
    • BTB/POZ domain-containing protein 6 antibody
    • BTBD 6 antibody
    • BTBD6 antibody
    • BTBD6_HUMAN antibody
    • Glucocorticoid receptor AF 1 coactivator 1 antibody
    • Lens BTB domain protein antibody
    • OTTHUMP00000163908 antibody
    see all

Anti-BTB/POZ domain containing protein 6 antibody images

  • Anti-BTB/POZ domain containing protein 6 antibody (ab66788) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling BTB/POZ with ab66788 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the villi. Magnification: 400X.

References for Anti-BTB/POZ domain containing protein 6 antibody (ab66788)

ab66788 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"