Overview

  • Product name
  • Description
    Rabbit polyclonal to BTF3
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 35-70 of Human BTF3 (NP_001032726.1).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      General transcription factor. BTF3 can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
    • Sequence similarities
      Belongs to the NAC-beta family.
      Contains 1 NAC-A/B (NAC-alpha/beta) domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic transcription factor 3 antibody
      • BETA NAC antibody
      • BTF 3 antibody
      • btf3 antibody
      • BTF3_HUMAN antibody
      • BTF3a antibody
      • BTF3b antibody
      • NACB antibody
      • Nascent polypeptide associated complex beta polypeptide antibody
      • RNA polymerase B transcription factor 3 antibody
      • Transcription factor BTF3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and human prostate carcinoma (right) tissues labelling BTF3 with ab113974 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of BTF3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab113974 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-BTF3 antibody which recognizes a upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

      Predicted band size : 22 kDa.

    References

    ab113974 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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