The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 23, 18 kDa (predicted molecular weight: 23 , 18 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionGeneral transcription factor. BTF3 can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
Sequence similaritiesBelongs to the NAC-beta family. Contains 1 NAC-A/B (NAC-alpha/beta) domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF3 antibody (ab66940)This image is courtesy of an abreview submitted by Aamir Ahmed, University College London, United Kingdom
IHC-P image of BTF3 (ab66940) on Testis sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 mins at 22°C.
Thakur D et al. Human Beta Casein Fragment (54-59) Modulates M. bovis BCG Survival and Basic Transcription Factor 3 (BTF3) Expression in THP-1 Cell Line. PLoS One7:e45905 (2012).
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