Anti-BTG1 antibody [2095C1a] (ab50991)

Overview

  • Product nameAnti-BTG1 antibody [2095C1a]
    See all BTG1 primary antibodies
  • Description
    Mouse monoclonal [2095C1a] to BTG1
  • Specificityab50991 has not yet been tested on endogenous protein in cell lysate.
  • Tested applicationsSuitable for: Dot blot, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of BTG1 (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab50991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 19 kDa.

Target

  • RelevanceBTG1 belongs to the BTG family of proteins.FUNCTION: Anti-proliferative protein. Its expression is associated with the early G1 phase of the cell cycle. A chromosomal aberration involving BTG1 may be a cause of a form of B cell chronic lymphocytic leukemia; translocation t(8;12)(q24;q22) with MYC.
  • Database links
  • Alternative names
    • B cell translocation gene 1 protein antibody
    • B cell translocation gene 1, anti proliferative antibody
    • BTG1 protein antibody
    • Protein BTG1 antibody

Anti-BTG1 antibody [2095C1a] images

References for Anti-BTG1 antibody [2095C1a] (ab50991)

ab50991 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (RS411 (B-cell leukemia))
Loading amount 100000 cells
Specification RS411 (B-cell leukemia)
Treatment 5µM MG132 over night
Gel Running Conditions Reduced Denaturing
Blocking step 2% milk + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Dec 01 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (RS411 (B-cell leukemia))
Specification RS411 (B-cell leukemia)
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde in EDTA/EGTA/H
Detection step Real-time PCR
Positive control ChIP on TBP and Glucocorticoid Receptor
Negative control Isotype control
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Abcam user community

Verified customer

Submitted Dec 01 2010

Thank you for your patience in this matter. The originator of ab50991 (ab50991 BTG1 antibody [2095C1a]) has provided the following information: The WB image was obtained under the following conditions: -Primary Ab dilution; 1:100 -Secondary Ab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"